Nucleus

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Welcome!

Nucleus is an open source package for synthetic cell builders maintained by b.next. Nucleus includes integrated digital tools, biological materials, documentation, and validated protocols for the full process of cell building, including cytosol construction, DNA content engineering, and membrane encapsulation.

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🎊What’s new in v0.2.1

The fourth stable release of Nucleus (v0.2.1) is now available. Nucleus is supported by Schmidt Futures and the California Institute of Technology.

Synthetic Cell

Booting up a simple synthetic cell

Container

Container Documentation

Container Protocols

Cytosol

Cytosol Documentation

Content

DNA Distribution

Development

All software development, module designs, and DNA are shared on Github: nucleus on GitHub.

PURE protein expression in pOpen vs pET28a

Cytosol

Liposome construction workshop at Caltech

Containers

The effect of DNA concentration on expression in liposomes

Cells

Community

Nucleus Community Contributions

About

Building synthetic cells that can sense and respond