This is the release of v0.4.0, which includes stable Nucleus content (i.e., passing our internal testing and generally working).

Release: 23 July 2025

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General

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• Nucleus is now Nucleus Distribution.
• The open science policy has been updated Open science.
• A dedicated FAQs page has been added FAQs.
• Developer Notes and Nucleus Community Contributions is being sunset on Nucleus Distribution) Developer Notes will now appear on a dedicated site at https://devnotes.bnext.bio/.
• A section for tutorials has been introduced that includes Nucleus Hub, Developer Notes, and Making Synthetic Cells for Beginners.
• The Development section is being renamed Software.

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• The “Hello, World” Liposome Protocol has been renamed PURE Cell and is now categorized as a Synthetic Cell.
• The protocol “Booting up a simple synthetic cell” has been renamed Making Synthetic Cells for Beginners and placed under Guides.

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• The OnePot PURE workshop module includes new DNA content encoding for the 36 PURE proteins in the pET28a backbone.

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• Make OnePot Protein Mix is added as an alternative to the existing 36-pot protocol for making PURE protein mix.
• Make Ribosomes introduces a protocol for purifying E. coli ribosomes from A19 biomass.
• Make tRNAs is updated to recommend purifying from A19 biomass over MRE600.
• The structure of PURE Protocols has been re-arranged for clarity.

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