Containers are the outsides of synthetic cells. Containers form a barrier between the interior (or “cytosols”) of synthetic cells and the environment, and are typically made of phospholipids forming a bilayer.
We currently use and recommend the phase-transfer method to prepare liposomes. It is simple, robust, and produces high quality liposomes at low throughput.
High density cytosol is first emulsified in lipids. This emulsion is then transferred to a low density outer solution and centrifuged, forming lipid droplets as the dense cytosol crosses the oil-aqueous interface.
The phase-transfer method imposes two constraints on the inner solution (or cytosol) and the outer solution:
- Inner solution must be more dense than outer solution.
- Inner solution must have the same osmolarity as outer solution.
Making LiposomesKey concepts and procedures for making liposomes using a phase-transfer technique.
Liposome workshop moduleDocumentation for running an educational two-day liposome construction workshop.
How to collect and analyze images of liposomes using light microscopy.
Container Protocols- Basic Components How to make the basic components (buffers, etc) required to make liposomes
- Making Liposomes How to encapsulate buffers and PURE into liposomes.
- Measuring Liposomes
How to measure and analyze liposomes.
- Liposome Analysis Tool—uses a CNN-based method to identify liposomes in microscopy images and calculate descriptive statistics.
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Containers |