Getting Started
You have a sample that is (nominally) fluorescent. You want to measure it’s fluorescence over time. You may even want to heat your sample while doing so. This protocol is for you.
Materials and Equipment
Protocol
(Optionally) prewarm plate reader to appropriate temperature (we used 37C).
Load between 10 uL and 50 uL of each sample onto a 384-well plate.
Seal plate with optically clear plate seals.
Set your plate reader settings. Here are example settings for measuring green fluorescence in a time series).
Excitation: 475 nm OR blue laser setting.
Emission: 510 nm OR green filter setting (generally between 500 nm and 550 nm).
Time between reads: 5 min.
Total number of reads: 100 (or 500 min / 8.3 hrs)
Run experiment!
Resources and References
Other Protocols
Papers
Resources
Credits
Yan Zhang, Zoila Jurado, and Miki Yun (Richard Murray Lab, Caltech)
Developers
Testers