PURE is a defined set of protein synthesis molecules widely used as the core of synthetic cell engineering. The system was originally developed in 2001 and made available commercially as PUREfrex (GeneFrontier Corporation) and PURExpress (NEB) at high cost and under proprietary formulation. Several protocols have since been published to make PURE production more accessible, including TraMOS PURE, PURE 3.0, and OnePot PURE. These protocols have generated significant interest but have proven surprisingly difficult to boot up. Reproducibility is limited by high biological variance intrinsic to existing protocols and DNA distributions as well as a lack of openly available and shareable DNA. Nucleus PURE is a clean sheet open source approach to make PURE construction easy, accessible, and reproducible.
⚠️ We’re still testing these protocols ⚠️
Nucleus PURE protocols are still in development. We’re still working on making these protocols work robustly & with our newly designed open-source PURE gene collection.
In the meantime, feel free to use these protocols. We cannot guarantee that they’ll work for you (yet) but we’re happy to share what we’ve learned here and answer any questions that you may have. Join our mailing list or